Cellular Imaging Microscope in Lake Nona

Cellular Imaging (Lake Nona)

Providing access to sophisticated microscopes for digital imaging.


The Cellular Imaging Lake Nona Facility broadly supports research programs by providing access to sophisticated microscopes for digital imaging, as well as training, assistance and guidance, helping scientists from any institution on a first come-first served basis.

The facility offers expertise, training and assistance in advanced biological microscopic imaging techniques and use of complex image processing software, use of well-maintained, aligned, and calibrated microscopic equipment, and troubleshooting of equipment and experimental problems.


Widefield Microscopy
  • Brightfield and Darkfield
  • Phase and Nomarski Differential Interference Contrast (DIC)
  • Epifluorescence
  • Evanescent Wave (TIRF)
  • Live Cell and Fixed Specimen
Confocal Microscopy
  • Point Scanning
  • Resonant Scanning
  • Spectral
  • VAAS Detection (Virtual Adaptable Aperture System)
  • Live Cell and Fixed Specimen
Electron Microscopy
  • Transmission
  • Digital Capture
  • Fixed Specimen
  • Image Analysis
  • Training and Consultation


  • Olympus InvertedIX71
    Brightfield, Phase and Widefield Fluorescence microscope with cooled, color and monochrome CCD camera (SPOT Pursuit 14 bit 1360x1024, Diagnostic Instruments). Runs on SPOT Advanced or Image Pro.
  • SoftwareFEI Morgagni Electron Microscope With CCD Camera
  • Nikon Inverted TIRF
    Brightfield, DIC and Widefield Fluorescence and Evanescent Wave (TIRF) fully motorized microscope with PFS (Perfect Focus System). Equipped for FRET and Ca2+ imaging, with a cooled monochrome EMCCD camera (QuantEM 16bit 512x512, Photometrics) and a cooled monochrome CCD camera (CoolSNAP HQ2 14bit 1392x1040, Photometrics). Lamdba DG-5 Xenon lamp, Mercury Intensilight, Ar-ion laser emitting at 488 and 514 nm and diode laser emitting at 561 nm. Runs on Nikon Elements Software.
  • NikonA1R VAAS
    Laser Point- and Resonant-Scanning Confocal Microscope equipped with single photon Ar-ion laser emitting at 457, 477, 488 and 514 nm; Diode lasers, emitting at 402, 561 nm, and 638 nm. 32-channel spectral detection and deconvolution, FRAP, FRET, photoactivation, high-speed resonant scanning or high-resolution point scanning, ultra-sensitive VAAS detector and Perfect Focus (PFS). Runs on Nikon Elements


For a Price List, please call (407)745-2000 or email us.


Research Specialist



Please call (407)745-2000 or use the button below to send us an email.

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