Histology Core Facility

Histology

Providing standard and customized research specific histology services including tissue processing, fixed and frozen sectioning.

 

The Histology Core Facility provides standard and customized research specific histology services including tissue processing, fixed and frozen sectioning. The Facility also offers routine and special histological stains as well as immunohistochemistry and immunofluorescence. The Facility maintains and provides training for several pieces of common use equipment, such as paraffin microtome, cryostat, and cytospin. Training is also provided for basic histological techniques such as perfusion and tissue collection. Finished slides are returned to the researcher for evaluation, or they may be digitized using our latest addition, the Aperio Scanscope. The Aperio Scanscope allows not only digitizing of slides for archiving, it also offers powerful image analysis tools as well. A tissue acquisition service assists investigators with regulatory/legal compliance, as well as obtaining and storage of human tissue samples and associated clinical data.

 

Conventional histology
  • Aperio high-resolution slide scanning and optional image analysis for bright field and fluorescence samples
  • Routine and custom sectioning
  • Paraffin and frozen samples
  • Standard and special stain (H&E, PAS, silver impregnation)
Immunohistochemistry
  • Double labeling of antibodies
  • Immunofluorescence
  • Development of custom protocols (overlay assays, competition assays)
  • Titration and staining of commercial and custom antibodies

 

  • Sakura Tissue Tek vacuum infiltration tissue processor
  • Aperio FL fluorescence scanner system
  • Aperio AT2
  • Leica CM 3050 cryostat
  • Leica RM 2125 paraffin microtome
  • Dako autostainer plus
  • Shandon Cytospin 3
  • Leica EG 1160 paraffin embedding station
  • MMI Cell Cut Laser Microdissection system

 

For a complete list of services, please call (858)646-3100 ext. 3552or email us.

Histology Services Internal
Subsidized
Internal External
Non-Profit
External
For-Profit
Small Combo/Embed/H&E/5unstained each $18 $22.50 $24.30 $47.34
Large Combo/Embed/H&E/10unstained each $28 $35 $37.80 $73.64
Additional Sections/Paraffin per slide $5.50 $6.88 $7.43 $14.47
Embedding Only/Paraffin each $6.50 $8.13 $8.78 $14.47
Immuno Staining (IHC or IF) per slide $35 $43.75 $47.25 $92.05
Aperio 20X scan per slide $10 $12.50 $13.50 $26.30
Aperio 40X scan per slide $20 $25 $27 $52.60
Aperio Fluorescence Scan per slide $20 $25 $27 $52.60
Common Cryostat per hour $22.20 $27.60 $30 $58.20

Leadership

Core Manager
Scientific Director

Contact

 

Guillermina (Gia) Garcia

Gia has been with Histology core for the past 8 years. She is responsible for the handling and processing of research samples on a day to day basis. She can answer any questions that you may have about your project. Gia is also skilled in a wide range of immunohistochemistry protocols, as well as a variety of histological staining methods. 

 

Please call (858)646-3100 ext. 3556 or use the button below to send us an email.
 

Contact the Facility

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